Figure 7
From: Loss of MafA and MafB expression promotes islet inflammation
TCR signaling gene expression in CD4+ and CD8+ T cells. (A,B) Heatmap showing Fluidigm gene expression from 100 pooled CD4+ and CD8+ T cells from 6–8 months-old pancreatic lymph nodes. (B) Drastic reduction in the expression of TCR signaling genes was observed in MafA−/−MafB+/− CD8+ T cells (N = 6–8 mice per genotype). Heatmap color gradient: dark red = highest expression; dark blue = lowest expression; white = intermediate; grey = no expression. (C,D) Flow cytometry analysis of wt and MafA−/−MafB+/− CD8+ T cells showing the percentage of Zap70+ (not stimulated) and (D) the fold increase in Zap70 (Y319) phosphorylation in response to acute stimulation with α-CD3 and α-CD28 antibody. (N = 5 mice/genotype; 4 independent experiments). Graphs are shown as mean ± SEM and analyzed with unpaired T-test with *P value ≤ 0.05 and **P ≤ 0.01 as significant.
