Figure 3 | Scientific Reports

Figure 3

From: Augmentation of spinal cord glutamatergic synaptic currents in zebrafish primary motoneurons expressing mutant human TARDBP (TDP-43)

Figure 3

Synaptic scaling of AMPA-mediated glutamatergic mEPSCs in primary motoneurons expressing mutTARDBP. (A) Example 10 second recordings of quantal AMPA-mediated glutamatergic mEPSCs recorded in CaP motoneurons. AMPA-mediated glutamatergic mEPSCs in mutTARDBP larvae were found to occur at an increased frequency when compared to either wild type larvae or larvae expressing wtTARDBP (B). The amplitude of mEPSCs recorded in CaP motoneurons expressing mutTARDBP were found to be significantly larger in amplitude when compared to larvae expressing wtTARDBP (C). Furthermore, when the entire recorded population of mEPSCs was examined, the cumulative probability of AMPA-mediated quantal events in larvae expressing mutTARDBP was found to be skewed right, indicating synaptic scaling (D). In addition, the half-width duration (E) and the rise time (F) of quantal events in larvae expressing mutTARDBP were found to be increased but no difference in the decay constant (G) was evident. Numbers in parentheses represent sample sizes. Asterisks and daggers represent statistical differences from wild type (p < 0.05) and wtTARDBP (p < 0.05) larvae respectively.

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