Figure 8 | Scientific Reports

Figure 8

From: CDK inhibitors reduce cell proliferation and reverse hypoxia-induced metastasis of neuroblastoma tumours in a chick embryo model

Figure 8

Hypoxia-triggered changes in gene expression is reversed by CDK1i in both BE(2)C and SKNAS tumours. (A) Comparison of gene expression between BE(2)C tumours formed from cells grown in 21% O2 and cells pre-cultured in 1% O2 for 3 days prior to implantation on the chick CAM at E7. (B) Comparison of gene expression between normoxia and DMOG-treated SKNAS tumours prepared as for BE(2)C tumours above. (C) Comparison between BE(2)C tumours formed from cells pre-cultured in 1% O2 for 3 days prior to implantation on the chick CAM at E7 and treated at E10 with either CDK1i or DMSO. (D) Comparison between SK-N-AS tumours formed from cells pre-treated with DMOG for 24 h prior to implantation on the chick CAM at E7 and treated at E10 with either CDK1i or DMSO. At least one tumour for each condition was obtained from a single experiment and used for comparison. mRNA levels were normalised to GAPDH, UBC and HPRT1 in each case and each bar in the graph represents the normalised mean ± SEM of at least three independent experiments. *P ≤ 0.05, **P ≤ 0.01 and ***P ≤ 0.001 compared with normoxia (A,B) or hypoxia (C,D).

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