Table 1 List of primer names and sequences for qPCR.
Gene | Gene name | Forward 5′-3′ | Reverse 5′-3′ |
|---|---|---|---|
GAPDH | glyceraldehyde-3-phosphate dehydrogenase | AATCCCATCACCATCTTCCA | TGGACTCCACGACGTACTCA |
HPRT1 | hypoxanthine phosphoribosyltransferase 1 | TGACACTGGCAAAACAATGCA | GGTCCTTTTCACCAGCAAGCT |
UBC | ubiquitin C | ATTTGGGTCGCGGTTCTTG | TGCCTTGACATTCTCGATGGT |
KLF4 | Kruppel-like factor 4 | CGCCGCTCCATTACCAAGAGC | CGGTCGCATTTTTGGCACTG |
MYCN | Neuroblastoma-derived v-myc avian myelocytomatosis viral related oncogene | CACAAGGCCCTCAGTACCT | ACCACGTCGATTTCTTCCTCT |
ROBO2 | roundabout, axon guidance receptor, homolog 2 | GATGTGGTGAAGCAACCAGC | TGGCAGCACATCTCCACG |
ADAMTS1 | ADAM metallopeptidase with thrombospondin type 1 motif,1 | CCCTCACTCTGCGGAACTTT | GGACCCACACAAGTCCTGTC |
GLUT1 | Glucose transporter 1, also SLC2A1 | GAACTCTTCAGCCAGGGTCC | ACCACACAGTTGCTCCACAT |
ITGαV | Integrin, alpha V | CGCTTCTTCTCTCGGGACTC | AGAAACATCCGGGAAGACGC |
ITGβ5 | Integrin, beta 5 | GGGAGCCAGAGTGTGGAAAC | GGATCGCTCGCTCTGAAACT |
MMP9 | Matrix metallopeptidase 9 | TTCTGCCCGGACCAAGGATA | ATGCCATTCACGTCGTCCTT |
VCAM | Vascular cell adhesion molecule 1 | TGTTTGCAGCTTCTCAAGCTTTTA | GTCACCTTCCCATTCAGTGGA |
VCAN | Versican | ACCAGACAGGCTTCCCTCCCC | GGGTGATGCAGTTTCTGCGAGGA |
VEGF | Vascular endothelial growth factor | CTCCACCATGCCAAGTGGTC | GCAGTAGCTGCGCTGATAGA |
