Figure 2

In ovo lineage trace of 3 Thrb CRMs yields unique patterns of activity. (a–e) Representative images of vertically sectioned retinas that were electroporated in ovo at E3 with CAG::nucβgal as an electroporation control, bp::PhiC31 (a), CAG::PhiC31 (b), ThrbCRM1::PhiC31 (c), ThrbCRM2::PhiC31 (d), or ThrbICR::PhiC31 (e) and CAaNa::GFP. Embryos were grown until E10, and all images are maximum intensity projections. To the right of each image is a schematic of the chick retina with recombined cell types from all counted images colored in green. (f) Quantification of the % recombination in each retinal layer, for each of the conditions assessed, n = 3–8. (g) Quantification of the % of representation for each retinal cell type among the recombined cells for that particular condition (GFP+ cells of one cell type/all GFP+ cells *100). The negative control had no recombination (f) so it was excluded here. Error bars represent SEM, n = 3–8. ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer; bp, basal promoter; PRs, photoreceptors; HCs, horizontal cells; ACs, amacrine cells; BCs, bipolar cells; RGCs, retinal ganglion cells.