Figure 4
The HCs in the ThrbCRM1 lineage are predominantly the H1 type. (a) Representative image of the INL of a ThrbCRM1 lineage traced flat-mounted retina at E10, counterstained with Lim1(white) to mark H1 HCs. Areas zoomed in insets are outlined in dotted line. Yellow arrows represent electroporated GFP+/Lim1+ cells, orange arrows represent electroporated GFP+/Lim1− cells. Electroporated βgal+ cells are shown in red, maximum intensity projection of 40x image. (b) Representative image of the INL of a ThrbCRM1 lineage traced flat-mounted retina at E10, counterstained with Islet1 (white) (DSHB, 39.3F7) to mark H2, H3 and H4 HCs. Areas zoomed in insets are outlined in dotted line. Yellow arrows represent electroporated GFP+/Islet1+ cells, orange arrows represent electroporated GFP+/Islet1− cells. Electroporated βgal+ cells are shown in red, maximum intensity projection of 40x image. (c) Quantification of Lim1 immunostained retinas as shown in a- % Lim1+ cells of electroporated cells in the HC layer of the INL (βgal+) and of ThrbCRM1 lineage traced INL cells (GFP+). Error bars represent SEM, n = 3. (d) Quantification of Islet1 immunostained retinas as shown in b- % Islet1+ cells of electroporated cells in the HC layer of the INL (βgal+) and of ThrbCRM1 lineage traced INL cells (GFP+). Error bars represent SEM, n = 3. (e–f) Representative images of a Lim1+ (e) or Islet1+ (f) HC within the ThrbCRM1 lineage. INL, inner nuclear layer; HC, horizontal cell.
