Figure 2

Establishment and characterization of human osteoblast spheroids: (A) Schematic representation of spheroids formation after aggregation and compaction of cell suspension cultured in nonadherent conditions (B) phase contrast images obtained by optical microscopy demonstrating the growth of spheroids produced with different numbers of cells (10000, 20000, 30000 and 50000 cells). (C) Spheroid diameter and volume is plotted against the different number of plated cells from three independent experiments (n = 10). Data are shown as mean ± SD. Spheroid diameter and volume was calculated based on phase contrast image analysis by area determination using image J software. A p-value < 0.05 is marked as statistically significant (***).