Figure 3
From: The two faces of titanium dioxide nanoparticles bio-camouflage in 3D bone spheroids
Spheroids characterization: (A) Cell viability of spheroids produced with different cell numbers measured by Dead Cell Apoptosis Kit in flow cytometry. Bars are shown as mean ± SD, calculated from a group of 12 spheroids for each condition. *P < 0.05 vs control. (B) Morphology of the spheroids produced with 10000 cells obtained by scanning electron microscopy where high magnification images demonstrate cell-cell interactions (B1). (C) Confocal microscopy of a live spheroid stained in red with ethidium-positive (dead cells) and in green with calcein-positive (live cells), merged image demonstrating the presence of live and dead cells. The fluorescence intensity profiles for both channels show the different distribution of live and dead cells in the spheroid structure.
