Figure 1
From: Identification of the fructose transporter GLUT5 (SLC2A5) as a novel target of nuclear receptor LXR
Transcriptional regulation of the human GLUT5 promoter by nuclear receptors LXR and THR. Luciferase reporter analysis of the hGLUT5 promoter for regulation by (a) Nuclear Receptors heterodimerizing with RXR; (b) THRα, THRβ; (c) LXRα and LXRβ. CV-1 cells were co-transfected with the proximal human GLUT5 promoter (−900/+3 bp) and expression plasmids for the different NRs in combination with equal amounts of heterodimeric partner RXRα. Y-axis indicates luciferase activity relative to LacZ and normalized to the basal hGLUT5 promoter activity. Ligand-dependent activation of THR and LXR was tested by addition of T3 (1 μM) or T09 (1 μM), respectively, at 24 h after transfection. (d) Dose-dependent stimulation of LXRα-mediated hGLUT5 promoter regulation by LXR ligand T0901317.
