Figure 2
From: Identification of the fructose transporter GLUT5 (SLC2A5) as a novel target of nuclear receptor LXR
Identification of the functional LXR-responsive element in the human GLUT5 promoter. (a) Overview of the promoter truncations used to localize the functional LXRE in the proximal human GLUT5 promoter (−900/+3 bp). Predicted LXREs are represented by green boxes. The red box represents a site-directed point mutation in the functional LXRE. (b) Luciferase reporter analysis of the hGLUT5 promoter truncations and mutant for regulation by LXR and THR. CV-1 cells were co-transfected with the indicated hGLUT5-luc constructs and expression plasmids for LXRα or THRα in combination with equal amounts of heterodimeric partner RXRα. X-axis indicates luciferase activity relative to LacZ and normalized to the negative control (NC = basal hGLUT5 promoter activity). Ligand-dependent activation of LXR and THR was tested by addition of T09 (1 μM) or T3 (1 μM), respectively, at 24 h after transfection. For statistics, different truncations/mutations were compared for each receptor in the presence of ligand.
