Figure 3
From: Identification of the fructose transporter GLUT5 (SLC2A5) as a novel target of nuclear receptor LXR
Transcriptional regulation of the mouse Glut5 promoter by nuclear receptors LXR and THR. Luciferase reporter analysis of the mGlut5 promoter for regulation by nuclear receptors LXRα, LXRβ, THRα and THRβ. CV-1 cells were co-transfected with the proximal mouse Glut5 promoter (−1014/+3 bp) and expression plasmids for the different NRs in combination with equal amounts of heterodimeric partner RXRα. Y-axis indicates luciferase activity relative to LacZ and normalized to the basal hGLUT5 promoter activity. Ligand-dependent activation of LXR and THR was tested by addition of T09 (1 μM) or T3 (1 μM), respectively, at 24 h after transfection.
