Figure 2 | Scientific Reports

Figure 2

From: Wnt signalling mediates miR-133a nuclear re-localization for the transcriptional control of Dnmt3b in cardiac cells

Figure 2

Inhibition of the Wnt/β-catenin signalling pathway induces an increase in AGO2 protein levels into the nucleus of HL-1 cells and its loading with miR-133a. (A) Western blot assay and densitometry for protein levels of AGO2 and AGO1 on three biological replicates of nuclear lysates (1-2-3;4-5-6) derived from HL-1 cells treated or not treated (NT) with IWR-1 (48 h). (For Western blot assay cropped image). (B) Western blot assay for immunoprecipitated endogenous AGO2 on isolated nuclei of HL-1 cells treated or not with IWR-1 (48 h), and qRT-PCR for miRNAs following endogenous AGO2 immunoprecipitation on isolated nuclei of HL-1 cells stimulated with IWR-1 (n = 3); ***p < 0.01. (For Western blot cropped images of parts of two gels).

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