Figure 3 | Scientific Reports

Figure 3

From: Wnt signalling mediates miR-133a nuclear re-localization for the transcriptional control of Dnmt3b in cardiac cells

Figure 3

AGO2 is required for the nuclear localization of miR-133a in HL-1 cells in response to IWR-1 treatment. (A,C) qRT-PCR for Ago2 and Ago1 mRNA levels after transient transfection of specific siRNAs. (B,D) qRT-PCR for mature miR-133a from isolated nuclei of HL-1 cells in presence or absence of IWR-1 (48 h). (E) Representative FISH image HL-1 cells transfected with of siRNAs against AGO2, AGO1 and SCR, and treated or not with IWR-1. Red, miR-133a; blue, DAPI. (F) Graphic plot of the average miR-133a signal over the nuclear area, in HL-1 cells transfected with siRNAs and treated or not with IWR-1 as indicated. Red, miR-133a; blue, DAPI (n = 6); *p < 0.05; **p < 0.01.

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