Figure 8
The nuclear re-localization of the AGO2/miR-133a complex is essential for differentiation of hESCs into cardiomyocytes. (A) Schematic representation of the cardiac differentiation protocol of hESCs. (B) qRT-PCR for miR-133a and DNMT3B on isolated nuclei and total cell lysate of differentiating hESCs. (C) qRT-PCR for DNMT3B on FITC+ sorted cells treated as indicated. (D) qRT-PCR on MHY6, TNNI2 and CACNA1C genes in hESC-CMs (n = 3); *p < 0.05; **p < 0.01; ***p < 0.001. (E) Immunostaining for al α-actinin and Cavα1.2 on hESC-CMs at day 15 of cardiac differentiation treated with FITC-conjugated TSB-miR or TSB-SCR.
