Figure 2

LMP1 induced the sumoylation of SENP2. (a) EBV-transformed LCLs were grown and denaturing immunoprecipitations (to pull-down all proteins covalently modified by SUMO-1) were done. (b) Denaturing immunoprecipitations were performed on lysates from paired EBV-negative and EBV-positive BL41 B-cells. (c,d) HEK 293 cells were transfected and denaturing immunoprecipitations were performed using antibodies specific to (c) endogenous SUMO-1 or its IgG isotype control or (d) exogenous myc-SUMO1. Western blot analyses were done to detect SENP2 (GFP), LMP1, and/or myc-SUMO1 levels.