Figure 5

The lht mutant shows a complete lack of vascular lumen. Confocal microscope images of the vascular system of WT and lht mutant embryos derived from Tg(fli1:GFP) medaka at 3 dpf (n > 10) (A–F). White arrows in A, C, and E indicate the vascular lumen. White arrowheads in B, D, and F show the absence of a vascular lumen. BCA, basal communicating artery; CaDI, caudal division of the primitive internal carotid artery; CrDI, cranial division of the primitive internal carotid artery; DA, dorsal aorta; DCV, dorsal ciliary vein; DLAV, dorsal longitudinal anastomotic vessel; ISV, intersegmental vessel; NCA, nasal ciliary artery; PCV, posterior cardinal vein; PHBC, primordial hindbrain channel; PMBC, primordial midbrain channel. Scale bars: 100 μm (A–F).