Figure 5 | Scientific Reports

Figure 5

From: Manassantin B shows antiviral activity against coxsackievirus B3 infection by activation of the STING/TBK-1/IRF3 signalling pathway

Figure 5

Manassantin B induced STING/TBK-1 signalling in coxsackievirus B3-infected cells. (a) Representative immunoblot of STING, p-STING, TBK-1, p-TBK-1, IRF-3, p-IRF-3, and β-actin to evaluate activation of their expression in coxsackievirus B3 (CVB3)-infected Vero cells or uninfected cells after treatment with manassantin B (Man B) (2 µg/mL). The samples were derived from the same experiment, and gels/blots were processed in parallel. (b) Density of western blot bands was calculated as the relative expression of STING, p-STING, TBK-1, p-TBK-1, IRF-3, and p-IRF-3. Each band was normalised by the density of the β-actin band. Data are presented as means ± SEM of 3 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 for comparison with non-infected control group (Ctrl); +P < 0.05, ++P < 0.01, +++P < 0.001 for comparison with CVB3-infected vehicle group; ###P < 0.001 for comparison with CVB3-infected Man B group, Bonferroni’s multiple comparison test (ANOVA).

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