Figure 1

Characterization of immune cells in the TPLN and HPLN of NOD mice. (a) Co-immunostaining of B220 (red) and CD3 (green) in whole region of sectioned PLNs. Histology magnification was 4x, scale bars represent 500 µm. Images are representative of three independent experiments (n = 3). (b) TPLN and HPLN sections from respectively 4, 8 and 12 wks old NOD mice were co-immunostained for LYVE-1 (red) and CD169⁺ subcapsular sinus macrophages (green). Histology magnification was 20X and scale bars represent 100 µm. Image are representative of three independent experiments (n = 3). (c,d) Representative flow cytometric analysis and quantitative bar graphs of the expression of CD11b⁺ macrophages and CD11c⁺ dendritic cells in the PLNs of 12 weeks old NOD mice. All data are expressed as mean ± SD, *p < 0.05, **p < 0.01.