Figure 2

Comparative analysis of the lymphatic vessels and HEVs of HPLN and TPLN. (a) HPLN and TPLN sections from respectively 4, 8 and 12 wks old mice were co-stained with LYVE-1 (red) and HEV (green). Histology magnification 4× and 20×, scale bars represent 500 µm and 100 µm. Images are representative of three independent experiments (n = 3). (b,c) quantitative bar graphs of Lyve-1⁺ staining and Meca⁺ in HPLN vs TPLN, the quantification were performed by ImageJ, data are representative of n = 3 images per group and 3 sections per each LN were analyzed. (d) Representative flow cytometric analysis and quantitative bar graph showing the expression of Lyve-1⁺ cells in the PLNs of 12 weeks old NOD mice. All data are expressed as mean ± SD, *p < 0.05, **p < 0.01.