Figure 3

Comparative analysis of FRCs and the ECM organization between TPLN and HPLN. (a) Whole scan images of representative sections of TPLN and HPLN from respectively 4, 8 and 12wks old NOD mice showing the staining for PDPN (red), Fibronectin (green) and Collagen IV (red). Histology magnification 4x and Scale bars represent 500 µm. Images are representative of three independent experiments (n = 3). (b–f) Quantitative bar graphs of PDPN⁺, fibronectin⁺, ERTR-7⁺, collagen IV⁺ and collagen I⁺ staining in HPLN vs TPLN, the quantification positive areas were performed by ImageJ, data are representative of n = 5 images per group and 3 mice per group were analyzed. All data are expressed as mean ± SD, *p < 0.05, **p < 0.01.