Figure 4
From: PSD-95 deficiency disrupts PFC-associated function and behavior during neurodevelopment
PSD-95 deficiency attenuates eEPSPs and normal temporal summation in layer V pyramidal neurons in the mPFC. (A) Schematic of layer II/III stimulation and whole-cell patch clamp recordings of layer V pyramidal neurons in the mPFC. (B) Representative traces of 10-pulse AMPAR-eEPSPs recorded at −70 mV from control and PSD-95−/−mice at postnatal day 35. (C) Summary graph of peak voltage (Vm) of eEPSPs (Repeated measures of ANOVA; F(1,26) = 8.23, p = 0.008, Con, n = 12, KO, n = 14). (D) Paired-pulse ratio (PPR) was calculated from the measured peak 2 Vm /peak 1 Vm (p = 0.42, n.s., Con, n = 12, KO, n = 14). (E) Summary graph of normalized peak (Vm) (Repeated measures of ANOVA; F(1, 24) = 0.34, p = 0.86, Con, n = 12, KO, n = 14). **p < 0.01.
