Table 4 In vitro and in vivo antioxidant activity of Nostoc sp. R76DM phycocyanin and its comparative account with other phycocyanin.

In vitro (Biochemical assay)

DPPH assay (% DPPH scavenging)

63.15 ± 0.85

71.10 ± 0.06

74.85 ± 2.14%

72.26 ± 1.21%

FRAP assay (Ascorbic acid equivalent antioxidant Capacity)

0.22 ± 0.01

0.29 ± 0.05

0.49 ± 0.04

0.46 ± 0.06

Reducing power assay (Ascorbic acid equivalent antioxidant Capacity)

0.40 ± 0.01

0.44 ± 0.03

0.53 ± 0.05

0.48 ± 0.04

In vivo (C. elegans)

DCFH-DA staining (% reduction in fluorescence intensity)

88.70

78.88

83.11

Not available

Protective effect against oxidative stress (Fraction survival in %)

84.04 ± 6.27

80.00 ± 7.07

67.2 ± 6.40

64.9 ± 5.10

Protective effect against thermal stress (Increase in fraction survival in %)

~36

~10

~25

Not available