Figure 4 | Scientific Reports

Figure 4

From: Identification of ryuvidine as a KDM5A inhibitor

Figure 4

Ryuvidine repressed H3K4 demethylation in HEK293 cells expressing Flag-tagged KDM5A. Flag-tagged KDM5A was transiently overexpressed in HEK293 cells and cells were treated with ryuvidineat 2 uM for 48 h. Flag and H3K4me3 were detected by immunostaining; DAPI staining indicates nuclei. Transfection of empty vector did not produce Flag-positive cells and H3K4me3 was clearly detected. H3K4me3 was markedly reduced in Flag-positive cells in which KDM5A was overexpressed. Ryuvidine repressed the reduction of H3K4me3.

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