Figure 2
Growth of L. monocytogenes ladR, lstR and lltR mutants. (a) L. monocytogenes strains EGD-e (wt), LMSH1 (ΔladR), LMSH2 (ΔlstR) and LMSH3 (lltRL49A R51A L52A) were grown in BHI broth at 37 °C. (b) Growth of the same set of strains at 6 °C. Growth curves were repeated three times and average values and standard deviations are shown. (c) Complementation of the cold-sensitive growth defect of the lltR mutant. L. monocytogenes strains EGD-e (wt), LMSH3 (lltRL49A R51A L52A) and the complemented strain LMSH42 (+lltR) were grown for six weeks on BHI agar containing 1 mM IPTG at 6 °C.
