Table 2 Strains and plasmids used in this study.
name | relevant characteristics | source*/reference |
|---|---|---|
plasmids | ||
pIMK3 | Phelp-lacO lacI neo | |
pMAD | bla erm bgaB | |
pBP117 | lacZ neo | |
pSAH1 | bla erm bgaB ΔladR | this work |
pSAH3 | bla erm bgaB ΔlstR | this work |
pSAH4 | bla erm bgaB lltR (lmo0599) | this work |
pSAH5 | bla erm bgaB lltR L49A R51A L52A | this work |
pSAH12 | PsigC-lacZ neo | this work |
pSAH14 | PmdrL-lacZ neo | this work |
pSAH15 | PlltR-lacZ neo | this work |
pSAH32 | bla erm bgaB Δlmo0421 | this work |
pSAH33 | bla erm bgaB lstR L90A L92A L93A | this work |
pSAH34 | bla erm bgaB lstRL90A L92A L93A Δlmo0421 | this work |
pSAH37 | Phelp-lacO-lltR lacI neo | this work |
pSAH45 | bla erm bgaB lltRL49A R51A L52A Δlmo0600 | this work |
pSAH46 | bla erm bgaB lltRL49A R51A L52A Δlmo0601 | this work |
pSAH62 | bla erm bgaB Δlmo2428-2427 | this work |
pSAH66 | bla erm bgaB ‘ftsW1’ | this work |
pSAH67 | bla erm bgaB ‘rodA3’ | this work |
pSAH68 | bla erm bgaB ‘ftsW2’ | this work |
pSAH69 | bla erm bgaB sigC* | this work |
L. monocytogenes strains | ||
EGD-e | wild type, serovar 1/2a strain | lab collection |
LMSH16 | attB::lacZ neo | |
LMSH1 | ΔladR (lmo1408) | pSAH1 ↔ EGD-e |
LMSH2 | ΔlstR (lmo0422) | pSAH3 ↔ EGD-e |
LMSH3 | lltR L49A R51A L52A | pSAH5 ↔ EGD-e |
LMSH10 | attB::PmdrL-lacZ neo | pSAH14 → EGD-e |
LMSH11 | ΔladR attB::PmdrL-lacZ neo | pSAH14 → LMSH1 |
LMSH12 | attB::PsigC-lacZ neo | pSAH12 → EGD-e |
LMSH13 | ΔlstR attB::PsigC-lacZ neo | pSAH12 → LMSH2 |
LMSH14 | attB::PlltR-lacZ neo | pSAH15 → EGD-e |
LMSH15 | lltRL49A R51A L52A attB::PlltR-lacZ neo | pSAH15 → LMSH3 |
LMSH39 | lstR L90A L92A L93A | pSAH33 ↔ EGD-e |
LMSH40 | lstRL90A L92A L93A Δlmo0421 | pSAH34 ↔ LMSH39 |
LMSH42 | lltRL49A R51A L52A attB::Phelp-lacO-lltR lacI neo | pSAH37 → LMSH3 |
LMSH50 | lltRL49A R51A L52A Δlmo0600 | pSAH45 ↔ LMSH3 |
LMSH51 | lltRL49A R51A L52A Δlmo0601 | pSAH46 ↔ LMSH3 |
LMSH63 | lstRL90A L92A L93A attB::PsigC-lacZ neo | pSAH12 → LMSH39 |
LMSH67 | Δlmo2428-2427 | pSAH62 ↔ EGD-e |
LMSH68 | lstRL90A L92A L93A Δlmo2428-2427 | pSAH62 ↔ LMSH39 |
LMSH89 | sigC* (lmo0423) | pSAH69 ↔ EGD-e |
LMSH90 | lstRL90A L92A L93A sigC* | pSAH69 ↔ LMSH39 |
LMSH96 | sigC* attB::PsigC-lacZ neo | pSAH12 → LMSH89 |
LMSH97 | lstRL90A L92A L93A sigC* attB::PsigC-lacZ neo | pSAH12 → LMSH90 |
