Figure 3
Evaluation of VOCs related with kindled seizures using principal component analysis (PCA). The principal component analysis (PCA) scores plot was derived from absolute values of 15 VOCs (A) and 13 VOCs (B) in the urine of amygdala-kindled and sham-operated mice. PC1 to PC3 scores of kindled mice (red circles), sham operated mice (blue circles), and PC1 to PC3 standardized scoring coefficients (black circles) in the principal component score coefficient matrix of each VOC were represented as the X-, Y-, and Z-axis, respectively. Promax rotation with Kaiser normalization was applied to the PCA with 13 VOCs, and components 1 to 3 of the pattern matrix of each VOC were represented as the X-, Y-, and Z-axis, respectively (C). A dendrogram of 13 VOCs was derived with the Ward method from the component scores 1 to 6 of each VOC (D). The vertical axis shows the number described in Table 1. Abbreviations: 2-acetyl-pyrroline, 2AP; 2-acetylpyrrole, APle; 2-butanone, 2B; 2-heptanone, 2H; 2-pentanone, 2P; acetophenone, Ac; 3,4-dehydro-exo-brevicomin, DEB; dimethyl trisulfide, DMTS; disulfide, dimethyl, DSDM; methane, nitro-, MN; methanethiol, MT; trimethylamine, TMA; 2,3,5-trithiahexane, TTH. The 6 VOCs include 2AP, 2H, Ac, APle, and MT, TTH in B; the 8 VOCs include the 6 VOCs in B and RI1227 and RI1449 in A.
