Figure 1 | Scientific Reports

Figure 1

From: Large-scale Discovery of Substrates of the Human Kinome

Figure 1

Overview of in vitro kinase profiling. HeLa cell lysate was dephosphorylated with alkaline phosphatase, and spiked phosphatase was inactivated with heat. Small aliquots of the dephosphorylated lysate were reacted with recombinant protein kinase. The mock-reacted sample was used as a control. After tryptic digestion, both samples were labeled with heavy or light formaldehyde. Phosphopeptides enriched with titania-HAMMOC14 were analyzed with nanoLC-MS.

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