Figure 2 | Scientific Reports

Figure 2

From: Human intestinal spheroids cultured using Sacrificial Micromolding as a model system for studying drug transport

Figure 2

Lumenization and polarization of intestinal spheroids after 6 days in culture. (A) A representative brightfield confocal image of a lumenized intestinal spheroid at day 6. (B) 3D reconstruction of a confocal z-stack showing a representative spheroid forming a confluent monolayer (DAPI) and a continuous actin belt (phalloidin) surrounding the apical membrane. (C) Fluorescence images of a brush border shown by ezrin (purple) co-localized with actin (green) on the apical membrane facing the lumen (10X). E-cadherins (red) are forming between cells showing cellular cross-communication while β-1 integrin (grey) is expressed on the basolateral membrane engaging with the extracellular matrix (10X).

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