Figure 2 | Scientific Reports

Figure 2

From: Precursor RNA processing 3 is required for male fertility, and germline stem cell self-renewal and differentiation via regulating spliceosome function in Drosophila testes

Figure 2

The function of Prp3 in spermatogonia. (A–D) Immunostaining of control, Bam > bam RNAi, Bam > Prp3 RNAi, and Bam > Prp3 RNAi; Δ86/+ testes. Representative point fusomes are indicated with white arrowheads and branch fusomes are indicated with yellow arrowheads. (E) The count of point fusomes in control, Bam > bam RNAi, Bam > Prp3 RNAi, and Bam > Prp3 RNAi; Δ86/+ testes. (F–M) Immunostaining of control, Bam > bam RNAi, Bam > Prp3 RNAi, and Bam > Prp3 RNAi; Δ86/+ testes. DNA was stained with Hoechst33342, and could label undifferentiated cells at the apex of the testis (yellow double arrowheads) and label the clusters of elongated spermatids at the tail of the testis (red arrowheads). (N) The count of elongated spermatid clusters. Data were analyzed by Student’s t test (all differences relative to control), *represents for P value < 0.05, **represents for P value < 0.01, ***represents for P value < 0.001. Error bars represent SEM. Scale bars: 20 µM.

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