Figure 3

Knockdown of Prp3 in spermatogonia caused germ cell differentiation defects. (A) Phase contrast view of wild-type testes. Spermatogenic cells at different stages (spermatogonia, spermatocytes, round spermatids, elongated spermatids, and mature sperm) could be identified. (B–D) Phase contrast view of the tip of the testis. Spermatogonia (yellow double arrowheads) accumulated in Prp3 RNAi and Prp3 RNAi; Δ86/+ testes compared with the control. Scale bars: 20 µM.