Figure 5 | Scientific Reports

Figure 5

From: Precursor RNA processing 3 is required for male fertility, and germline stem cell self-renewal and differentiation via regulating spliceosome function in Drosophila testes

Figure 5

Prp3 knockdown caused cell death in Drosophila S2 cells. (A) Relative Prp3 mRNA level in control and Prp3 siRNA (Prp3 siRNA-354 and Prp3 siRNA-1660) cells to validate knockdown efficiency. Prp3 siRNA-354 was more efficient and used in following experiments. (B) Immunostaining of control and Prp3 RNAi using TUNEL and DNA (Hoechst). (C) Percentage of TUNEL-positive cells in control and Prp3 RNAi. (D) Flow cytometry testing of control and Prp3 RNAi. Ratio of live (Q4), apoptotic (Q2 + Q3) and necrotic (Q1) cells were calculated at different intervals. (E) Percentage of cell components in control and Prp3 RNAi. (F) CCK-8 assay for control and Prp3 RNAi. Student’s t test was used for the statistical analysis. *represents for P value < 0.05, **represents for P value < 0.01, ***represents for P value < 0.001. Error bars represent SEM. Scale bar: 30 μM.

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