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Figure 1

From: Fast dose fractionation using ultra-short laser accelerated proton pulses can increase cancer cell mortality, which relies on functional PARP1 protein

Figure 1

Dose responses of DNA damage foci formation and of cell survival. (A) Representative immune-fluorescent images of SF763 cells obtained 1 h and 24 h after exposure to the indicated doses of laser driven protons. The negative controls (0 Gy) were sham-irradiated. Merged images show γH2AX (green) and DNA (blue). (B, C) Number of γH2AX foci induced by laser driven protons (LDP, dotted square), conventional accelerated protons (CAP, triangles) and X-rays (x cross) respectively in SF763 and U87-MG cells. Each data point represents the mean of at least three independent experiments in which at least 300 nuclei were analyzed and averaged. (D, E) Normalized cell survival resulting from exposure to increasing doses of LDP, CAP and X-rays respectively in SF763 and U87-MG cells. Each data point represents the mean and standard deviation (SD) of three replicates obtained at least with three independent experiments. Survival curves were generated following the linear quadratic model (R > 0.96 and R > 0.97 for SF763 and U87-MG cell lines respectively).

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