Figure 4 | Scientific Reports

Figure 4

From: Fast dose fractionation using ultra-short laser accelerated proton pulses can increase cancer cell mortality, which relies on functional PARP1 protein

Figure 4

Impact of PARP1 inhibition on cell survival oscillation in response to the variation of the delay between LDP bunches. (A) Western Blot detection of the PARP1 protein and parylation in total protein extracts from HCT116 WT cells left untreated, treated 10 min with 1 mM hydrogen peroxide (H2O2) or treated one hour with 200 nM Olaparib before H2O2 treatment. (B) HCT116 WT cells were left untreated (white circles) or treated one hour with 200 nM Olaparib (black circles) and were then exposed to five bunches of LDP (3.5 ± 0.77 Gy). Each data point represents the mean and SD of three replicates obtained in two independent experiments. Comparisons of the cell survival were performed using by two way ANOVA multiple comparisons test (Tukey’s multiple comparisons test).

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