Figure 5 | Scientific Reports

Figure 5

From: Micro RNAs upregulated in Vitiligo skin play an important role in its aetiopathogenesis by altering TRP1 expression and keratinocyte-melanocytes cross-talk

Figure 5

Expression of TRP1 in lesional (L), non-lesional (NL) epidermis and Normal human epidermal keratinocytes (NHEK). (a) Transcriptional expression of TRP1 shown as ΔCT in non-lesional and lesional epidermis from vitiligo patients. (n = 8) (b) Transcriptional expression of TRP1 shown as ΔCT in NHEK (n = 10) and lesional epidermis (n = 10) from vitiligo patients, (c) Downregulation of TRP1 expression in terms of fold change in lesional epidermis compared with non-lesional epidermis in 8 Vitiligo patients samples (VD). (d) Western blot for expression of TRP1 in four samples of non-lesional vs. lesional epidermis and normal human epidermal keratinocytes (678 K) at protein level showing lower expression in lesional epidermis. Full gel pictures are shown in Supplementary Fig. 14. (e) Densitometric analysis for expression of TRP1 in Non-lesional vs. Lesional epidermis normalized with endogenous control COX-IV. (f) Densitometric analysis for expression of TRP1 in Keratinocytes (678 K) vs. Lesional epidermis. All the bars showing reduced expression are depicted as mean ± S.E.M.

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