Figure 6 | Scientific Reports

Figure 6

From: Micro RNAs upregulated in Vitiligo skin play an important role in its aetiopathogenesis by altering TRP1 expression and keratinocyte-melanocytes cross-talk

Figure 6

Melanosome transfer from melanocytes to keratinocytes after transfection of keratinocytes with selected pre-miRs. (a) Representative image for pellet color in pure Keratinocyte culture (NHEK, with no pigment) and keratinocytes transfected with miRNAs, co-cultured with melanocytes and differentially trypsinized to get only pure keratinocytes. Pellet color is lighter in transfected Keratinocytes co-cultured with Melanocytes as compared to scrambled control (NC). UT is Untransfected control. (b) Bar graph showing percentage decrease in Melanin content in keratinocytes that were transfected with, pre-miR-202, pre-miR-518a-5p and pre-miR-525-5p, co-cultured with melanocytes and obtained 48 hours post co-culture by differential trypsinization. As compared to the scrambled  control i.e. NC (100%, 8.03 ± 3.1 µg), miR-202 showed 76.32 ± 7.6% (6.48 ± 3.1 µg), miR-518a showed 73.2 ± 8.4% (6.21 ± 2.98 µg) and miR-525 showed 67.75 ± 7.3% (5.89 ± 2.8 µg) melanin content (c) Bar graph showing absolute melanin content in the keratinocyte cultures mentioned in (b) All the bars showing reduced expression are depicted as mean ± S.E.M mentioned above.

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