Figure 2 | Scientific Reports

Figure 2

From: Intrabody-based FRET probe to visualize endogenous histone acetylation

Figure 2

Time-lapse FRET visualization of H3K9ac in live U2OS cells. (a) FRET images with 405 nm excitation. After incubation with 1 μM TSA, accumulation of probe in the nucleus and increase in FRET efficiency can be observed clearly. (b) Average of FRET index in time-lapse quantification: ratio of 520 nm F.I. (CFP) to 477 nm F.I. (YFP) with 405 nm excitation in nucleus (blue) and in cytoplasm (red). The FRET index in the cytoplasm remained steady while that in the nucleus rose because H3K9 acetylation level increased.

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