Figure 4
From: Intrabody-based FRET probe to visualize endogenous histone acetylation
Time-lapse visualization of H3K9ac levels in HeLa cells using a mintbody. Confocal images of HeLa cells stably expressing Venus-based H3K9ac-mintbody were acquired at 0 and 6 h after addition of 1 μM TSA. The relative nucleus/cytoplasmic ratio (averages from 30 cells with the standard deviations (a) and representative images (b) are shown.
