Figure 2
From: A behavior-based drug screening system using a Caenorhabditis elegans model of motor neuron disease
Evaluation of dnc-1 KD worms as a model for drug screening by MWT. (a) Representative trajectories of N2 (upper four groups) and dnc-1 KD worms (lower four groups) analyzed in the same assay plate by WMT. Moving speeds are depicted as a linear rainbow-color gradient. (b) The average moving speeds of each region (N2-1 to 4: light gray bars; dnc-1 KD-1 to 4: dark gray bars). (c) The average moving speed of N2 and dnc-1 KD worms (N2, n = 120; dnc-1 KD worms, n = 121). (d) Representative histogram of moving speeds of N2 and dnc-1 KD worms. (e) Statistical analysis and evaluation of dnc-1 KD worms for the drug screening by MWT. The Z’-factor is defined as: Z’-factor = 1–3(SD_N2 + SD_dnc-1 KD)/(Ave._N2-Ave._dnc-1KD). The statistical analysis in C was performed using the Student t-test (***p < 0.0001).
