Figure 5
From: A behavior-based drug screening system using a Caenorhabditis elegans model of motor neuron disease
Effects of nifedipine on the locomotion and axonal pathology of dnc-1 KD worms. (a) Dose-dependent effects of nifedipine on the locomotion of worms (n = 205, 170, and 230 for 0, 30, and 100 μM of nifedipine, respectively). (b) Histograms of the moving speed of control and nifedipine-treated dnc-1 KD worms. (c) Direct comparison of the effect of riluzole and nifedipine (n = 180, 210, and 170 for control, riluzole, and nifedipine). (d,e) The effects of riluzole and nifedipine on the pathology of dnc-1 KD worms. The number of axonal spheroids per transverse axon was counted after treatment by each compound (n = 20 worms for each group) (d), and representative images of a transverse axon with and without treatment by nifedipine are shown (e). Asterisks are axonal spheroids and arrowheads are the cell body of ventral motor neurons (e). (f) Number of axonal spheroids in a transverse axon treated by the hit compounds of the MWT assay (n = 20 worms for each group). Statistical analyses were performed by one-way ANOVA followed by the Bonferroni/Dunn post hoc test (a,c,d,f). (*p < 0.05, **p < 0.001, and ***p < 0.0001). Error bars indicate the S.E.M.
