Figure 1

Ormeloxifene inhibits cell proliferation and motility. (A) Ormeloxifene decreases cellular proliferation of Caski and SiHa cells. Caski and SiHa cells were treated with ormeloxifene (10, 20, 25 µM) for 48 hours and MTS method was used to determine proliferation and absorbance was measured at 490 nm. Results were normalized to the vehicle control (ETOH). Error bars show SEM, n = 3. *p < 0.05. (B) Growth kinetics through xCELLigence RTCA. Caski and SiHa cell lines were treated with 20 µM ormeloxifene and growth kinetics (rate of real time proliferation) was measured. (C,D) Ormeloxifene inhibits clonogenic potential of cells. (C) Cells showed inhibited colony forming ability after 15 days of ormeloxifene treatment. Results were normalized to the ETOH control. Error bars show SEM, n = 3. *p < 0.05. (D) Qualitative representation of inhibited clonogenecity of cells. Images were taken at 200X. (E) Ormeloxifene decreased the cellular migration and invasion. Cells were treated with ormeloxifene for 24 hours and images were taken at 100X. Both cells show clear inhibition of motility and invasion confirmed by Boyden chamber method. (F) Motility kinetics through xCELLigence RTCA. Real time migratory and invasive properties of Caski and SiHa cells were also confirmed using xCELLigence system.