Figure 4

Effects of ormeloxifene on HPV infection. Ormeloxifene decreases (A) HPV E6 and (B) E7 mRNAs. Caski cells were treated with ormeloxifene for 6 hours and mRNA levels of HPV E6 and E7 were determined by quantitative PCR. Expression levels were normalized to a house keeping gene (β-2-Microgloblin) and scaled to the vehicle control (ETOH). Bars = Relative Expression Level, Error bars show SEM, n = 3. *p < 0.05. (C) Ormeloxifene inhibits HPV E6 and E7 proteins and upregulates tumor suppressor proteins. Caski cells were treated with ormeloxifene for 24 hours and western blots were preformed to detect HPV E6 and E7, wt-p53, Rb and PTPN13 proteins. β -actin was used as a loading control. All full-length blots are presented in Supplementary Information. Band quantifications of (D) E6, (E) E7, (F) wt-p53, (G) Rb and (H) PTPN13. Band quantitation was done by using GelQuant software. Band intensity was normalized to β -actin and scaled to the ETOH control. Bars = Relative Expression Level, Error bars show SEM, n = 3. *p < 0.05.