Figure 1
Immunohistochemical detection of autophagy markers in liver tissues from patients with cirrhosis. (A,B) Paraffin-embedded three consecutive liver sections were stained with antibodies against LC3B, p62 or normal IgG. Ductular reaction in cirrhotic liver (B) showed strong expression of LC3B but were negative for p62, whereas hepatocytes in cirrhotic liver were positive for both LC3B and p62. Rabbit normal IgG was used as a negative control counterstain. Asterisks indicate the ductular reaction in cirrhotic livers. Scale bar, 50 μm. (C) Higher magnifications of the ductular reaction in corresponding cirrhotic liver sections are enlarged micrographs from the respective boxed area. The dotted lines indicated the same region among three consecutive sections. Scale bar, 20 μm.
