Figure 5
From: Modified CLARITY Achieving Faster and Better Intact Mouse Brain Clearing and Immunostaining
Images of passive cleared A4P0B0-processed brain. (a) The image was acquired by confocal microscopy (20x/0.75 dry objective). Dopaminergic neurons stained with Anti-Tyrosine Hydroxylase antibody. (Red: anti-TH; blue: DAPI; Scale bar: 50 μm; square width: 50 μm). (b) The image shows neurons in the white boxed region of figure a. (Scale bar: 20 μm; square width: 10 μm). (c) The image shows neuronal dendrites in figure b. It is reconstructed with the filament auto-path toolkit in Imaris 9.0.1 software (Bitplane) (Scale bar: 20 μm; square width: 10 μm).
