Table 1 Overview of targeting vector assembly, mESC targeting and aggregation.
GOI | pRMCE-DV3 targeting vector | RMCE targeting | #Aggregations | #Chimeras | Germline transmission |
|---|---|---|---|---|---|
LacZ | 2/5 | 12/12 | — | — | — |
Jarid2 | 5/5 | 8/8 | 5 | 8 | Yes |
dnETV6 | 5/5 | 8/8 | 1 | 5 | Yes |
Runx2 | 5/5 | 8/8 | 1 | 4 | Yes |
MN1 | 5/5 | 8/8 | 2 | 12 | Yes |
Total | 22/25 (88%) | 44/44 (100%) |
