Figure 3
(A–M) Legend: IHC staining for CD68/CD21 and CD3/CD20 on ST of patients with Rheumatoid Arthritis inadequately responder to MTX based on the BMI category. (A,B) Example photos of CD68(red)/CD21(brown) staining of ST biopsies from MTX-IR RA with BMI < 25 kg/m2; (C,D) Example photos of CD3(red)/CD20(brown) staining of ST biopsies from MTX-IR RA with BMI < 25 kg/m2; (E,F) Example photos of CD68(red)/CD21(brown) staining of ST biopsies from MTX-IR RA with BMI ≥ 25 kg/m2; (G,H) Example photos of CD3(red)/CD20(brown) staining of ST biopsies from MTX-IR RA patients with BMI ≥ 25 kg/m2; (all magnifications 20X) (I) Rate of follicular synovitis in MTX-IR RA with BMI < 25 Kg/m2 (50.0%) vs MTX-IR RA with BMI ≥ 25 Kg/m2 (48.0%; p = 0.97); (J) Lining and sublining IHC score for CD68 cells in ST of MTX-IR RA based on BMI category; Lining and sublining CD68 IHC score of overweight/obese vs normal weight MTX-IR RA (p = 0.95 and p = 0.90 respectively); (K) Lining and sublining IHC score for CD21 cells in ST of MTX-IR RA based on BMI category; Lining and sublining CD21 IHC score of overweight/obese vs normal weight MTX-IR RA (p = 0.89 and p = 0.79 respectively); (L) Lining and sublining IHC score for CD20 cells in ST of MTX-IR RA based on BMI category; Lining and sublining CD20 IHC score of overweight/obese vs normal weight MTX-IR RA (p = 0.92 and p = 0.38 respectively); (M) Lining and sublining IHC score for CD3 cells in ST of MTX-IR RA based on BMI category; Lining and sublining CD3 IHC score of overweight/obese vs normal weight MTX-IR RA (p = 0.80 and p = 0.49 respectively); RA: Rheumatoid Arthritis; MTX-IR: inadequately responder to Methotrexate; ST: Synovial Tissue; IHC: Immunohistochemistry; BMI: Body Mass Index; CD: Cluster Designation.
