Table 1 Characteristics of genes, primers, PCR-conditions, nucleotide diversity and polymorphic sites for the K. variicola MLST scheme.
From: Molecular epidemiology of Klebsiella variicola obtained from different sources
Locus | Function | Primer name | Primer sequence | Temp (°C) | Size (bp) | No. of alleles | Nucleotide diversity | Polymorphic sites (nonsynonymous substitutions) |
|---|---|---|---|---|---|---|---|---|
leuS | Leucyl-tRNA synthetase | leuSKv-F | CGAACAGGTTATCGACGGCT | 63 | 594 | 48 | 0.012462 | 48 (8) |
leuSKv-R | CAAAGGTGTCGGTTTCACGC | |||||||
pgi | Phosphoglucose isomerase | pgiKv-F | AAAGAGACCGATCTGGCAGG | 60 | 600 | 42 | 0.016425 | 49 (6) |
pgiKv-R | ACCAGATACCGATCAGCGCC | |||||||
pgk | Phosphoglycerate kinase | pgkKv-F | TCGTGATGGATGCTTTCGGT | 63 | 444 | 26 | 0.012647 | 33 (10) |
pgkKv-R | AGATTTTGTCAGCGATGCCG | |||||||
phoE | Phosphoporine E | phoEKv-F | CTGTACGACGTGGAAGCCTG | 63 | 453 | 54 | 0.018569 | 46 (10) |
phoEKv-R | CCACGAAGGCGTTCATGTTT | |||||||
pyrG | CTP synthase | pyrGKv-F | CCGATCGCTATGGTCGCTG | 60 | 522 | 60 | 0.011535 | 48 (22) |
pyrGKv-R | CGGGACATCAGTTCCGGGT | |||||||
rpoB | β-subunit of RNA polymerase B | rpoBKv-F | GCCAGCTGTCCCAGTTTATG | 60 | 513 | 25 | 0.014945 | 38 (3) |
rpoBKv-R | GAACGGTACCGCCACGTTTA | |||||||
fusA | Elongation factor G | fusAKv-F | CGAAAACCAAAGCTGACCAGG | 62 | 561 | 15 | 0.007130 | 23 (4) |
fusAKv-R | CATGGTGTATGATGCACGACCT |
