Figure 1

Internalization of otopathogenic S. aureus into HMEECs. HMEECs were infected with four clinical strains of S. aureus at different multiplicity of infection (MOI) and then subjected to gentamicin and lysostaphin protection assay to determine bacterial cell invasion (A). In separate experiments, HMEECs were infected with S. aureus at a MOI of 10 for different post-infection time periods and bacterial colonization was determined (B). Data represents mean ± standard deviation and is representative of five different experiments carried in triplicate.