Figure 2 | Scientific Reports

Figure 2

From: Otopathogenic Staphylococcus aureus Invades Human Middle Ear Epithelial Cells Primarily through Cholesterol Dependent Pathway

Figure 2

Invasion of HMEECs by S. aureus visualized through confocal laser scanning microscopy. HMEECs were left uninfected or infected with S. aureus for 1 h and 2 h followed by staining with anti-S. aureus antibody. Samples were then stained with a secondary Alexa Fluor® 488 antibody (green) and counterstained with DAPI (blue) to visualize cell nuclei. Cells were then subjected to microscopy. Yellow arrows indicate bacteria. Results are representative of five independent experiments carried out in triplicate. Scale bars: 5 µm.

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