Figure 3

UmRrm75 gene expression analysis in FB2 parental strain under thermal, osmotic, and oxidative conditions. (A) FB2 parental strain grown for 24 h in liquid MM at 15 °C, 28 °C and 37 °C. (B) FB2 strain grown for 24 h in liquid MM with 1 M sorbitol or 1 mM H₂O₂. Transcript levels of UmRrm75 were calculated by the qRT-PCR approach. Normalized fold change was calculated comparing the UmRrm75 gene expression (under stress condition) with control conditions, after normalization to the UmGAPDH using the (2^−ΔΔCt) method. Analyses were performed in triplicate. Data represent the mean ± SEM. One-Way Analysis of Variance (ANOVA) and Tukey’s post-test analyses were performed. Significant differences are marked with an asterisk (P < 0.05).