Figure 8
Catalase treatment and enzyme activity, and UmYap1 expression in the ΔUmRrm75 mutant and parental strains. (A) Determination of catalase (CAT) activity in FB2 parental and 1/46 mutant strains at 10, 12 and 24 h. Data are means ± SEM from three biological replicates (n = 3). The different letters indicate a significant difference according to One-Way Analysis of Variance (ANOVA) and Tukey’s post-test analysis (P < 0.05). (B) Exogenous application of CAT in ΔUmRrm75 mutant strains. Cultures grown in liquid MM with DCFH2-DA at 28 °C; Left panel (without CAT), and right panel (250 U/mL CAT). Images were acquired on a fluorescence microscope at 40× (oil-objective) magnification. One representative microscopy image of DCFH2-DA fluorescence is shown from three biological replicates. (C) Transcript level analysis of UmYap1 gene in FB2 parental and 1/46 mutant strains. Normalized fold change was calculated by comparing the UmYap1 gene expression in 1/46 mutant with the FB2 parental, after normalization to UmGAPDH using the (2−ΔΔCt) method. Analyses were performed in triplicate. Unpaired t tests were performed and data represent the mean ± SEM. Significant differences are marked with an asterisk (P < 0.0001).
